微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pARO181 |
|---|---|
| 商品货号： | TS154951 |
| Designations： | pARO181 |
| Depositors： | D Parke |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0700001716613770 Vector: pARO181 (plasmid) Promoters: Promoter lac Construction: pUC18, pSUP2021, Tn5 Marker(s):kanR Construct size (kb): 6.070000171661377 Features: insert detection: lacZ marker(s): kanR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector permitting visual detection of recombinants vector useful for insertional mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/PvuI--6.0; PvuI--6.0; EcoRI/BglII--3.7, 2.3; EcoRI/PvuI--6.0. Mobilizable expression vector encoding the lacZalpha (lacZ) peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Replicates to high copy number in E. coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. The order of the major features in this plasmid is: lacZ/MCS - pMB1 ori - kanR - oriT/bom. pARO181 (TS154951) and pARO191 (77126) differ in the orientation of the MCS in lacZ. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of E. coli containing the plasmid |