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| 产品名称： | pJK210 |
|---|---|
| 商品货号： | TS155010 |
| Designations： | pJK210 |
| Depositors： | JB Keeney |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0339999198913570 Vector: pJK210 (plasmid) Promoters: Promoter lac Construction: pJK142 Marker(s):ampR,ura4+ Construct size (kb): 5.033999919891357 Features: insert detection: lacZ marker(s): ampR marker(s): ura4+ promoter: lac replicon: pMB1 |
| Applications： | contains sequence dihydroorotase integrating vector shuttle vector vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.9; EcoRV--4.9; HindIII--2.6, 1.7, 0.8. Shuttle vector for integrating sequences at ura4. Permits visual detection of recombinants by lacZalpha complementation. Constructed from pJK142 (ATCC 86955) by inserting the ura4 gene at the NdeI site. The ura4 gene contains the following unique restriction sites: BsmI BsgI PflMI Bsu36I StuI BbsI AvrII. The order of the major feature in the plasmid is: ampR - pMB1 ori - 5 lacZ - SacI/MCS/KpnI - 3 lacZ - ura4. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439 Jill B Keeney, personal communication |
| Related Products： | component of:ATCC 86958 component of:ATCC 87686 |
| Shipped： | frozen |
| Shipping Information： | xa0Freeze dried Escherichia coli containing the plasmid. |