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购买须知
| 产品名称： | 93T449 |
|---|---|
| 商品货号： | TS155035 |
| Organism： | Homo sapiens, human |
| Tissue： | retroperitoneum |
| Product Format： | frozen |
| Morphology： | fibroblast-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | liposarcoma; well-differentiated |
| Age： | 68 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The MDM2 amplification present in this cell line can be used as a model for testing new drugs. This cell line is one of the rare liposarcoma cell lines available. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 49~53,xx,+3;7r cp8/49~52,XX,-7,-22,+4~7r, +mar6/q8~106, idemx2cp11.ish r mar (1;4;10;amp12q14-15;15) (wcpl+; wcp 4+; wcp 10+, wcp 12+, amp MDM2, amp CDK4; wcp 15+). rev ish amp (1q24’ 1q31, 4p16, 10p15, 12q14, 12q21, 15q26) |
| Images： |  |
| Derivation： | The 93T449 cell line was established from a well-differentiated liposarcoma from the retroperitoneum of a 68 year old female. |
| Oncogene： | MDM2 (murine double minute 2); CDK4 (cyclin-dependent kinase 4); HMGA2 (high mobility group AT-hook 2) |
| Comments： | Karyotypic and molecular characterization of this liposarcoma demonstrated the presence of ring and large marker chromosomes containing MDM2, CDK4 and HMGA2 amplification. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | CSF1PO: 10, 11 D13S317: 8xa0 D16S539: 12, 13 D5S818: 12 D7S820: 10 TH01: 8, 9 TPOX: 8, 11 vWA: 14, 17 Amelogenin: X |
| Name of Depositor： | F Pedeutour |