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| 产品名称： | Influenza A virus (H3N2) |
|---|---|
| 商品货号： | TS155062 |
| Classification： | Orthomyxoviridae, Alphainfluenzavirus |
| Agent： | Influenza A virus (H3N2) |
| Strain： | A/Aichi/2/68 |
| Applications： | Emerging infectious disease research Respiratory research Influenza Research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived by adaptation from allantoic product ATCC® VR-547™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-547™ and ATCC® VR-1680™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture. |
| Effect on Host： | CPE, rounding and degeneration |
| Recommended Host： | MDCK (ATCC® CCL-34™) |
| Growth Conditions： | Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
| Name of Depositor： | MT Coleman, Centers for Disease Control and Prevention; ATCC® |
| Source： | Isolated from sailor on Israeli ship docking in Aichi, Japan, 1968 |
| References： | Coleman MT, et al. The Hong Kong-68 influenza A2 variant. Lancet 2: 1384-1386, 1968. PubMed: 4177941 |