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| 产品名称： | pSRA-2 |
|---|---|
| 商品货号： | TS155086 |
| Designations： | pSRA-2 |
| Species： | Rous sarcoma virus |
| Depositors： | J Bishop |
| Vector： | Construct size (kb): 13.69999980926514 |
| Insert： | DNA: genomic Insert lengths(kb): 9.399999618530273 Tissue: DNA from quail QT-6 cell line |
| Insert Size (kb)： | 9.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 4.3, 1.7; EcoRI--8.2, 3.0, 2.5; SalI--9.4, 4.3. The clone was derived from unintegrated DNA from an acute infection of QT-6 cells. Unintegrated DNA isolated from an acute infection of QT-6 cells by SRA-2 virus. Permuted SRA-2 molecule from lambda gt WES was circul A gag-specific probe can be generated from a 1.35 kb BamHI fragment; a pol-specific probe can be generated from a 0.8 kb HindIII/BamHI fragment; an LTR-specific probe from a 0.32 kb EcoRI fragment, and a src-specific probe from a 0.8 kb PvuII fragment. SRA-2 from lambdagtWES (linearized at SacI) was circularized, cut with SalI, and cloned into pBR322. |
| Classification： | Retrovirus, Avian Type C Retrovirus Group |
| References： | DeLorbe WJ, et al. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J. Virol. 36: 50-61, 1980. PubMed: 6255214 J. Michael Bishop, personal communication |