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微信小章| 产品名称： | Bak KO SV40 MEF |
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| 商品货号： | TS155094 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryonic fibroblast |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0 Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, BAK function, as well as cells that are resistant to multiple apoptotic stimuli. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Cells are immortalized MEFs, which were generated from BAK knockout mice. |
| Tumorigenic： | no |
| Comments： | Cells are immortalized MEFs (genotype: bak-/-), which were generated from BAK knockout mice. Cells are sensitive to tBID-induced cytochrome c release and apoptosis. Cells are sensitive to multiple apoptotic stimuli that act through disruption of mitochondrial function: staurosporine, ultraviolet radiation, growth factor deprivation, etoposide, and the endoplasmic reticulum stress stimuli thapsigargin and tunicamycin.xa0 Cells are sensitive to Bim and Bad expression induced apoptosis. BAK is a pro-apoptotic BCL-2 family member. This cell line is useful for studying molecular mechanism of cell apoptosis, BCL-2 family pathway, BAK function, as well as cells that are resistant to multiple apoptotic stimuli. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Cryopreservation： | Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | Stanley Korsmeyer |
| References： | Wei M, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292(5517): 727-730, 2001 PubMed: 11326099 Lindsten T, et al. The Combined Functions of Proapoptotic Bcl-2 Family Members Bak and Bax Are Essential for Normal Development of Multiple Tissues. Molecular Cell 6(6): 1389-1399, 2000 PubMed: 11163212 Zong WX, et al. BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev. 15(12): 1481-6, 2001 PubMed: 11410528 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631 |