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购买须知
| 产品名称： | pBSVH167 |
|---|---|
| 商品货号： | TS155126 |
| Designations： | pBSVH167 |
| Species： | Mus musculus, mouse |
| Depositors： | U Storb |
| Vector： | Construct size (kb): 2.700000047683716 |
| Insert： | DNA: genomic Insert lengths(kb): 0.1889999955892563 Tissue: myeloma MOPC-167 cell line Gene product: immunoglobulin heavy-chain variable region IghV |
| Insert Size (kb)： | 0.189 |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.4, 0.23; SmaI--uncut; EcoRI--2.7; BamHI--2.7; HindIII--2.7; EcoRI/BamHI--2.5, 0.2. The antibodies encoded by myeloma MOPC-167 are specific to phosphorylcholine. The insert was subcloned from ChM167alpha10.1. A 210 bp fragment can be excised by an EcoRI/BamHI double digest, which cuts in the polylinker. The vector pBSc was constructed by inserting the 382 bp PvuII fragment from pBluescribe (Stratagene) into pACYC184 (pUC8CmR) which had been partially digested with PvuII. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Storb U, et al. Trangenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies. J. Exp. Med. 164: 627-641, 1986. PubMed: 2425036 Ursula Storb, personal communication |