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微信小章| 产品名称： | RPTEC/TERT1 |
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| 商品货号： | TS155213 |
| Organism： | Homo sapiens, human |
| Tissue： | Renal cortex; proximal tubules, epithelium |
| Cell Type： | Epithelial cells immortalized with pLXSN-hTERT retroviral transfection |
| Product Format： | frozen 1.0 mL |
| Morphology： | Epithelial-like |
| Culture Properties： | Adherent |
| Biosafety Level： | 2 xa0Cells contain SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | Adult |
| Gender： | Male |
| Applications： | These cells are proposed to be a valuable model system not only for cell biology, but also toxicology, drug screening, biogerontology and tissue engineering.
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| Storage Conditions： | Liquid nitrogen vapor phase |
| Images： | 
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| Antigen Expression： | Antigen expression: This cell line is positive for epithelial marker pan-cytokeratin (immunocytochemistry)(verified at ATCC), positive for epithelial cell adhesion molecule E-cadherin (immunocytochemistry) (verified at ATCC),xa0 The RPTEC/TERT1 cells express both Aminopeptidase N (verified at ATCC) and γ-Glutamyl Transferase (GGT) that are located in the brush border of the renal proximal tubular epithelium. |
| Comments： | The RPTEC/TERT1 cells specifically respond to parathyroid hormone (PTH) but not arginine vasopressin (AVP), and react with enhanced ammonia genesis on lowering of the environmental pH.
The RPTEC/TERT1 cells exhibit sodium-dependent uptake of phosphate as well as intact functionality of the megalin/cubilin transport system.
RPTEC/TERT1 cells show the characteristic morphology and functional properties of normal proximal tubular epithelial cells.
At high cell densities, the RPTEC/TERT1 cells form characteristic "domes", maintain ultrastructural organization with tight junctions, densely packed microvilli and primary cilium, indicating functional cell polarization.
When cultured on the Corning™ Transwell™ Permeable membrane cell culture insert, the RPTEC/TERT1 cells at confluence form intact functional barrier as indicated by stabilized Trans-Epithelial Electrical Resistance (TEER) across the membrane.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium (ATCC® 30-2006™). To make the complete growth medium, add hTERT RPTEC Growth Kit (ATCC® ACS-4007™) to the base medium. The final concentration for each growth kit component in the complete hTERT immortalized RPTEC growth medium is as follows: xa0
Required but not supplied:xa0G418 solution MUST be added to the above medium to a final concentration of 0.1 mg/mL G418 to maintain the selective pressure for immortalization Note: Do not filter complete medium. This medium is formulated for use with a 5% CO2 in air atmosphere.
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| Subculturing： | Volumes are given for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended.
Medium renewal: 2 to 3 times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Aminal Cells: A Manual of Basic Techniques by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: DMEM:F12, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Volume： | 1.0 mL |
| STR Profile： | CSF1PO: 11
D13S317: 11, 13
D16S539: 11, 12
D5S818: 9, 11
D7S820: 10
THO1: 9, 9.3
TPOX: 8, 11
vWA: 16, 18
Amelogenin: XY |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
| Name of Depositor： | R Grillari-Voglauer |
| Year of Origin： | July 2004 |
| References： | Wieser M, et al. hTERT alone immortalizes epithelial cells of renal proximal tubules without changing their functional characteristics. Am. J. Physiol. Renal Physiol. 295: 1365-1375, 2008. PubMed: 18715936 Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Freshney RI. Culture of Animal Cells: A Manual of Basic Technique, 5th edition. New York: Wiley Liss; 2005. For more information on enzymatic dissociation and subculturing of cell lines see Chapter 13. Limonciel A, et al. Comparison of base-line and chemical-induced transcriptomic responses in HepaRG and RPTEC/TERT1 cells using TempO-Seq. Arch Toxicol 92(8):2517-2531, 2018. PubMed: 30008028 Simon-Friedt BR, et al. The RPTEC/TERT1 Cell Line as an Improved Tool for In Vitro Nephrotoxicity Assessments. Biol Trace Elem Res 166(1):66-71, 2015. PubMed: 25893367 Aschauer L, et al. Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1. Toxicol In Vitro 30(1 Pt A):95-105, 2015. PubMed: 25500123 Simon BR, et al. The RPTEC/TERT1 cell line models key renal cell responses to the environmental toxicants, benzoapyrene and cadmium. Toxicol Rep 1:231-242, 2014. PubMed: 25126521 Secker PF, et al. RPTEC/TERT1 cells form highly differentiated tubules when cultured in a 3D matrix. ALTEX 35(2):223-234, 2017. PubMed: 29197217 Shrestha S, et al. Human renal tubular cells contain CD24/CD133 progenitor cell populations: Implications for tubular regeneration after toxicant induced damage using cadmium as a model. Toxicol Appl Pharmacol 331:116-129, 2017. PubMed: 28587817 Simon BR, et al. Cadmium alters the formation of benzoapyrene DNA adducts in the RPTEC/TERT1 human renal proximal tubule epithelial cell line. Toxicol Rep 1:391-400, 2014. PubMed: 25170436 Soodvilai S, et al. Interaction of pharmaceutical excipients with organic cation transporters. Int J Pharm 520(1-2):14-20, 2017. PubMed: 28131852 Shah H, et al. Gene expression study of phase I and II metabolizing enzymes in RPTEC/TERT1 cell line: application in in vitro nephrotoxicity prediction. Xenobiotica 47(10):837-843, 2017. PubMed: 27616666 Kramer NI, et al. Biokinetics in repeated-dosing in vitro drug toxicity studies. Toxicol In Vitro 30(1 Pt A):217-24, 2015. PubMed: 26362508 Aschauer L, et al. Application of RPTEC/TERT1 cells for investigation of repeat dose nephrotoxicity: A transcriptomic study. Toxicol In Vitro 30(1 Pt A):106-16, 2015. PubMed: 25450743 Wilmes A, et al. Mechanism of cisplatin proximal tubule toxicity revealed by integrating transcriptomics, proteomics, metabolomics and biokinetics. Toxicol in Vitro 30(1 Pt A):117-27, 2015. PubMed: 25450742 Wilmes A, et al. Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress. J Proteomics V79:180-194, 2013. PubMed: 23238060 Wilmes A, et al. Evidence for a role of claudin 2 as a proximal tubular stress responsive paracellular water channel. Toxicol Appl Pharmacol 279(2):163-72, 2014. PubMed: 24907557 Jennings P, et al. Interleukin-19 as a translational indicator of renal injury. Arch Toxicol 89(1):101-6, 2015. PubMed: 24714768 Ranninger C, et al. Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High Performance Liquid Chromatography-Mass Spectrometry-based Experimental and Computational Pipeline. J Biol Chem 290(31): 19121-32, 2015 PubMed: 26055719 |