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微信小章| 产品名称： | Human enterovirus 71 |
|---|---|
| 商品货号： | TS155249 |
| Classification： | Picornaviridae, Enterovirus |
| Deposited As： | Enterovirus 71 |
| Agent： | Human enterovirus 71 |
| Strain： | H |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Effect on Host： | CPE, refractile rounding and sloughing |
| Recommended Host： | LLC-MK2 (ATCC® CCL-7.1™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 2 to 6 days |
| Name of Depositor： | ZM Zheng, National Cancer Institute |
| Source： | Vesicular fluid from an adult female with hand, foot, and mouth disease, Wuhan,xa0China |
| References： | Zheng ZM, et al. First isolation of enterovirus type 71 from vesicle fluid of an adult patient with hand-foot-mouth disease in China. Virologica Sinica. 4: 375-382, 1989. Zheng ZM, et al. Comparison of enterovirus 71 (E71) isolated from a patient with hand-foot-and-mouth disease in China to prototype E71 BrCr strain by polymerase chain reaction using a unique primer pair. Clin. Diagn. Virol. 1: 137-139, 1993. PubMed: 15566727 Zheng ZM, et al. Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5-noncoding region. J. Med. Virol. 47: 161-167, 1995. PubMed: 8830120 |