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| 产品名称： | yRM2003 HTY275 |
|---|---|
| 商品货号： | TS155253 |
| Designations： | yRM2003 HTY275 |
| Species： | Homo sapiens, human |
| Depositors： | HC Riethman |
| Vector： | Construct size (kb): 130.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pTYAC1 Intact vector size: 9.000 Type of vector: YAC Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI Polylinker sites: Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): restriction site: EcoRI other: telomere, Tetrahymena, marker(s): URA3 centromere: CEN4 replicon: ARS1 replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D2S446 Gene name: DNA Segment, single copy Contains complete coding sequence?: U Chromosome: 2; Localization: 2 q37 Genome: Homo sapiens Gene symbol: D2Z4 Gene name: DNA Segment, repetitive (telomere) Contains complete coding sequence?: U Chromosome: 2; Localization: 2 q37-qter Tissue: leukocyte Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 120.0 Cross references: Insert lengths(kb): 120.0 Tissue: leukocyte Gene product: DNA Segment, repetitive (telomere) D2Z4 |
| Insert Size (kb)： | 120.0 |
| Media： | AHC- (AHC plus 40 mg/L tryptophan)
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen |
| Comments： | Contains at least two CpG islands in the duplicated subtelomeric DNA approximately 100 kb from the telomere. This sequence is also available in the cosmid 2003 cosmid 1 (ATCC 99432). The insert of yRM2003 (TS155253) is contained within the insert of yRM2112 (ATCC 99418). |
| References： | Macina RA, et al. Sequence organization of the human chromosome 2q telomere. Hum. Mol. Genet. 3: 1847-1853, 1994. PubMed: 7545974 Riethman HC, et al. Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector. Proc. Natl. Acad. Sci. USA 86: 6240-6244, 1989. PubMed: 2668959 |