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| 产品名称： | pMMB33 JD50 |
|---|---|
| 商品货号： | TS155285 |
| Designations： | pMMB33 JD50 |
| Depositors： | J Frey |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.75 Vector: pMMB33 (cosmid) Construction: RSF1010, pHC79 Marker(s):kanR Construct size (kb): 13.75 Features: marker(s): kanR replicon: R300B |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A broad host range cosmid vector. It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB34, ATCC# 37249, only by the orientation of the 5.7 kb fragment. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Frey J, et al. Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24: 299-308, 1983. PubMed: 6315541 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the cosmid |