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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS155333 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | MC1061p3 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
| Preceptrol®： | no |
| Genotype： | F- delta(ara-leu) delta(lacIPOZY)X74 araD139 galE15 galK16 hsdR rpsL lambda- (p3 kanR amp(Am) tet(Am)) |
| Type Strain： | no |
| Comments： | Host for suppressor tRNA-containing plasmids. MC1061p3 has amber mutations in the genes conferring amp and tet resistance. It is an exconjugant of MC1061 with W3110p3. |
| Medium： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C Duration: The above host transformed with PcEXV-D2 plasmid should be selected with Amp 50/Tet 15 |
| Name of Depositor： | HV Huang |
| References： | . . Nucleic Acids Res. 11: 24-27, 1983. Lutz CT, et al. Syrinx 2A: An improved lambda phage vector designed for screening DNA libraries by recombination in vivo. Proc. Natl. Acad. Sci. USA 84: 4379-4383, 1987. PubMed: 2955406 |