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| 产品名称： | pHuR98 pHuR 98 |
|---|---|
| 商品货号： | TS155379 |
| Designations： | pHuR98 pHuR 98 |
| GenBank Number： | M20262 |
| Depositors： | RK Moyzis |
| Vector： | Construct size (kb): 4.60
Name of vector: pBR322Intact vector size: 4.36 Type of vector: plasmid Vector end: PstI Vector end: PstI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: Genomic
Insert lengths (kb): 0.16
Tissue: Placenta
Gene product: DNA Segment, repetitive D9Z3
Genome: humanGene symbol: D9Z3 Contains complete coding sequence?: U Chromosome: 9; Localization: 9 q12 Insert end: Modification: GC tails Cross references: DNA Seq. Acc.: M20262 |
| Insert Size (kb)： | 0.158 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; HindIII--4.5; NdeI--4.5; PstI--4.3, 0.21. DNA containing this sequence is underrepresented in most genomic libraries because most restriction enzymes (except HinfI and TaqI) generate fragments with pHuR98 elements larger than 20 kb. The repeat sequence of this member of the satellite 3 family consists of GAACCCGAGT followed by diverged GGAAT repeats. This insert contains repeat spacing of 54 and 45 nt, separated by a 29 nt repeat. |
| References： | Moyzis RK, et al. Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma 95: 375-386, 1987. PubMed: 3677921 |