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| 产品名称： | K7M2-pCI Neo |
|---|---|
| 商品货号： | TS155413 |
| Organism： | Mus musculus, mouse |
| Tissue： | Derived from metastatic site: lung |
| Cell Type： | osteoblast |
| Product Format： | frozen |
| Morphology： | osteoblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells containing CMV and SV40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Strain： | BALB/c |
| Applications： | tumor model |
| Images： |  |
| Derivation： | Antigen Expression: CD31 + PubMed:1315100Products: factor VIII PubMed: 11315100; integrin binding sialoprotein (BSP) PubMed11315100; biglyan PubMed: 11315100; decorrin PubMed:1315100; villin 2 (ezrin) PubMed: 11325848. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a naïve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line PubMed:11315100. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
| Genes Expressed： | decorin,secreted phosphoprotein 1 (osteopontin),villin 2 (ezrin),Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
| Cellular Products： | decorin Ref   Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100secreted phosphoprotein 1 (osteopontin) Ref Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100villin 2 (ezrin) Ref Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in BALB/c mice with spontaneous metastasis to the lungs in over 90% of mice inoculated Ref Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 |
| Comments： | Antigen Expression: CD31 + PubMed:1315100 Products: factor VIII PubMed: 11315100; integrin binding sialoprotein (BSP) PubMed11315100; biglyan PubMed: 11315100; decorrin PubMed:1315100; villin 2 (ezrin) PubMed: 11325848. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. The K7M2 wt cell line was derived by intra-osseous injection of the K7 murine osteosarcoma cell line to the proximal tibia of a BALB/c mouse. The resultant primary tumor spontaneously metastasized to the lungs. A pulmonary metastasis was removed from the lung and then surgically implanted to a paraosteal tibial muscle flap of a naïve mouse. This cycle of implantation of pulmonary metastasis to paraosteal tibial muscle flap sites was repeated, resulting in the K7M2 primary tumor. This tumor was dissociated to a single cell suspension to create the K7M2 wt cell line PubMed:11315100. Expression of bone sialoprotein, biglyan, decorrin, and osteopontin is suggestive of bone lineage cells. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.8 mg/ml G-418 and 10% fetal bovine serum
|
| Subculturing： | Protocol:
Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 1 X 10(5) cells/sq. cm Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Two to three times weekly |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Population Doubling Time： | 12 hours |
| Name of Depositor： | C Khanna |
| Year of Origin： | 2004 |
| References： | Khanna C, et al. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin. Exp. Metastasis 18: 261-271, 2000. PubMed: 11315100 Khanna C, et al. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res. 61: 3750-3759, 2001. PubMed: 11325848 Khanna C, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat. Med. 10: 182-186, 2004. PubMed: 14704791 The K7M2-pCI Neo cell line was derived from the K7M2 wt (ATCC CRL-2836) cell line. K7M2 wt cells were transfected with the pCI Neo vector. The vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. It also contains SV40 viral sequences and the neomycin resistance gene. |