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| 产品名称： | pMS-TRP |
|---|---|
| 商品货号： | TS155439 |
| Designations： | pMS-TRP |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8280000686645510 Vector: pMS-TRP (phagemid) Promoters: Promoter GAL Marker(s):TRP1,ampR Construct size (kb): 6.828000068664551 Features: marker(s): TRP1 marker(s): ampR other: Myristoylation site other: lox site promoter: GAL promoter: T7 replicon: 2 micron ori replicon: f1 replicon: pMB1 MCS: EcoRI...SphI restriction site: NotI terminator: CYC1 epitope tag: 3x hemaglutinin (HA) |
| Applications： | vector component of two-hybrid screening system |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.0, 2.8; BglI/XhoI--4.5, 2.3; SphI--6.8. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. Vector for use the the SOS Recruitment System (SRS). It encodes the v-Src myristoylation signal and can be used to construct expression libraries to be screened for interacting proteins. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808 |
| Shipped： | freeze-dried |