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| 产品名称： | DFF-45 |
|---|---|
| 商品货号： | TS155442 |
| Designations： | DFF-45 |
| GenBank Number： | U91985 |
| Species： | Homo sapiens, human |
| Depositors： | X Wang |
| Applications： | in suitable host, produces protein DNA fragmentation factor, 45 kDa subunit |
| Vector： | Construct size (kb): 5.579999923706055 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.679999947547913 Gene product: DNA fragmentation factor, 45 kDa subunit DFF45* Target Gene: DNA fragmentation factor, 45 kDa subunit |
| Insert Size (kb)： | 1.680 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): Uncut: EcoRI--3.9, 1.7; BamHI--4.6, 1.0; PvuII--2.6, 1.4, 0.9, 0.4, 0.3. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--1.04; BglI--0.00, 1.47; BglII--0.45; KpnI--0.08; NdeI--1.30; NcoI--1.47; PstI--0.57, 0.63, 0.94, 1.01; PvuII--0.44, 0.63; StuI--0.19, 0.92, 0.99; SacI--0.93. |
| References： | Liu X, et al. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 89: 175-184, 1997. PubMed: 9108473 |