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微信小章| 产品名称： | Human adenovirus 3 |
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| 商品货号： | TS155467 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus B |
| Deposited As： | Adenovirus type 3 |
| Agent： | Human adenovirus 3 |
| Strain： | GB |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Agglutinates rhesus monkey red blood cells. Weakly oncogenic, contains soluble group-specific CF antigen. |
| Effect on Host： | CPE, rounding, clumping, and sloughing |
| Recommended Host： | A549 (ATCC® CCL-185™) |
| Growth Conditions： | Temperature: 37°C
Recommendations For Infection:xa0Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days
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| Name of Depositor： | RJ Huebner, National Institute of Allergy and Infectious Diseases |
| Source： | Nasal washings from an adult male with a common cold, Maryland, 1953 |
| References： | Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828 Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955. |