Construct size (kb): 4.599999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAP1-PEN
Gene name: adenylyl cyclase-associated CAP protein, yeast homolog
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: L12168; EST: EST07211
Nucleotides 1-214 of the insert correspond to
nucleotides 2578-2365 of L12168 with 97% identity.
DNA Seq. Acc.: L12168; EST: EST07212
Nucleotides 1-351 of the insert correspond to
nucleotides 1127-1477 of L12168 with 98% identity.
DNA Seq. Acc.: T09318; EST: EST07211; Primer: M13-21; THC
id: THC123085
DNA Seq. Acc.: T09319; EST: EST07212; Primer: M13 Reverse;
THC id: THC123085

Insert lengths(kb): 1.399999976158142

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: adenylyl cyclase-associated CAP protein, yeast homolog CAP1-PEN

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 1.2; HindIII/NotI--3.0, 1.4.