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| 产品名称： | Amphora roettgeri Lee and Reimer |
|---|---|
| 商品货号： | TS155742 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | endosymbiont from foraminiferan, Heterostegina depressa, coral gardens in the northeastern part of Heron-Wistori Channel, southern extremity of the Great Barrier Reef, 1981 |
| Product Format： | frozen |
| Type Strain： | yes |
| Medium： | ATCC® Medium 1966: Soil seawater medium for algae |
| Growth Conditions： | Temperature: 25.0°C Protocol: ATCCNO: 50213 SPEC: Transfer thawed contents of ampule to a centrifuge tube, centrifuge at 300-400 x g for 5 minutes and remove 0.75 ml. Resuspend the cells in approximately 1 ml of fresh medium and transfer to a T-25 tissue culture flask containing 9 ml of fresh medium. Incubate under light intensity of approximately 50-75 uEinsteins/m2/sec. Subculture every month as follows: Rub the surface of the agar with a sterile cotton swab and aseptically transfer a 0.1 ml aliquot to a fresh flask of medium. |
| Subcultivation： | Protocol: ATCCNO: 50213 SPEC: Transfer thawed contents of ampule to a centrifuge tube, centrifuge at 300-400 x g for 5 minutes and remove 0.75 ml. Resuspend the cells in approximately 1 ml of fresh medium and transfer to a T-25 tissue culture flask containing 9 ml of fresh medium. Incubate under light intensity of approximately 50-75 uEinsteins/m2/sec. Subculture every month as follows: Rub the surface of the agar with a sterile cotton swab and aseptically transfer a 0.1 ml aliquot to a fresh flask of medium. |
| Name of Depositor： | JJ Lee |
| Year of Origin： | 1981 |
| References： | type strain |