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| 产品名称： | LL0YNL02 |
|---|---|
| 商品货号： | TS155752 |
| Designations： | LL0YNL02 |
| Species： | Homo sapiens, human |
| Depositors： | P De Jong, JA Garnes |
| Origin： | hybrid J460-51 cell line |
| Vector： | Tissue: hybrid J460-51 cell line Chromosome: Y DNA: genomic Digest: Partial Insert size range: 15 kb average Genome Equivalent: 15.0 Coverage: Clones for "complete" coverage: 5000.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen (volume: 0.2 ml) |
| Comments： | Purity (human clones): 83%. Contaminants (hamster): 4%. The J640-51 cell line was provided by C. Jones, Eleanor Roosevelt Institute of Cancer Research, Denver, CO 80206. It is described in Cytogenet. Cell Genet. 28: 181-194, 1980 and Somat. Cell. Mol. Genet. 17: 117-136, 1991. Chromosomes for library construction were isolated by Ger van der Engh using flow-sorting. Genomic equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 58 Mb/Y chromosome. |
| References： | Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608 Pieter De Jong, personal communication |