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| 产品名称： | pEMBL18-Not (Sma-) |
|---|---|
| 商品货号： | TS155808 |
| Designations： | pEMBL18-Not (Sma-) |
| Depositors： | A Craig |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9000000953674320 Vector: pEMBL18-Not (Sma-) (phagemid) Promoters: Promoter lac Construction: pEMBL18 Marker(s):ampR Construct size (kb): 3.900000095367432 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1, f1 |
| Applications： | vector for constructing genomic libraries vector permitting construction of fusion proteins vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.9; NotI--4.0; EcoRI--3.9. General purpose vector modified to include a NotI site to facilitate library construction and chromosome walking. SmaI site in polylinker of pEMBL18 changed to a NotI site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651 |
| Shipped： | freeze-dried |