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| 产品名称： | p423 GAL1 |
|---|---|
| 商品货号： | TS155811 |
| Designations： | p423 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.4889998435974120 DESCRIPTION OF VECTOR: Intact vector size: 6.489 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS423, GAL1 promoter Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): HIS3, ->, 200-1395 terminator: CYC1, ->, 2077-2337 MCS: XhoI...SpeI, ->, 2337-2394 promoter for expression: GAL1, marker(s): ampR, replicon: 2 micron, ->, 5042-6382 Vector: p423 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS423, GAL1 promoter Marker(s):ampR,HIS3 Construct size (kb): 6.488999843597412 Features: marker(s): HIS3 marker(s): ampR promoter for expression: GAL1 replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.45; KpnI--5.5, 1.0; PstI--5.3, 1.2. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |