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| 产品名称： | pTKbeta |
|---|---|
| 商品货号： | TS155903 |
| Designations： | pTKbeta |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 7.5999999046325680 DESCRIPTION OF VECTOR: Intact vector size: 7.600 Type of vector: plasmid Cloning sites: XbaI NotI SalI SphI HindIII Polylinker sites: EcoRI BamHI XbaI SalI PstI SphI HindIII Construction: pUC19, pC4AUG beta-gal, pL1, pXT1 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: HSV TK replicon: pMB1 terminator: SV40 late Vector: pTKbeta (plasmid) Promoters: Promoter HSV TK Construction: pUC19, pC4AUG beta-gal, pL1, pXT1 Marker(s):ampR Construct size (kb): 7.599999904632568 Features: insert detection: lacZ marker(s): ampR promoter: HSV TK replicon: pMB1 terminator: SV40 late |
| Applications： | contains easily purifiable cassette(s) for construction reporter construct vector for other uses vector permitting visual detection of activity in colonies |
| Comments： | Restriction digest of the clone give the following sizes (kb): EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5, 2.7, 0.9; NotI--4.4, 3.2; BamHI--3.8 (doublet). Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. Designed to express low levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. The lacZ gene may be excised by NotI and replaced with alternative sequences. The order of the major features in this plasmid is: pUC19 - EcoRI - HSV thymidine kinase promoter - SV40 late 16S/19S splice signals - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |