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| 产品名称： | p519gfp |
|---|---|
| 商品货号： | TS155953 |
| Designations： | p519gfp |
| Depositors： | AE Goodman, AG Matthysse |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.0000000000000000 Vector: p519gfp (plasmid) Promoters: Promoter for expression lac Marker(s):kanR Features: marker(s): kanR, neoR other: mob promoter for expression: lac MCS: HindIII/.../XbaI restriction site: EcoRI restriction site: NdeI ribosome-binding site: GFP coding sequence: GFPmut2 |
| Applications： | contains easily purifiable cassette(s) for construction green fluorescent protein (mutant) vector permitting visual detection of activity in colonies green fluorescent protein (mutant) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--9.0; EcoRI--9.2; EcoRI/HindIII--6.7, 1.7, 0.7; PstI--5.6, 1.8, 1.2. The promoterless reporter GFPmut2 cassette, with its ribosome binding site, can be recovered as a ndIII/EcoRI (5/3) fragment (approximately 740 bp). A broad host range mob+ plasmid with GFPmut2 expression controlled by a lac promoter. Cloning into the SphI, PstI, AccI, HincII, SalI, XbaI, or NdeI sites will probably affect GFPmut2* expression as it would separate the CDS or RBS from the lac promoter. This has not yet been tested. |
| Media： | ATCC® Medium 1634: LB medium (ATCC medium 1065) with 50 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Matthysse AG, et al. Construction of GFP vectors for use in gram negative bacteria other than E. coli. FEMS Microbiol. Lett. 145: 87-94, 1996. PubMed: 8931331 Keen NT, et al. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197, 1988. PubMed: 2853689 Cormack BP, et al. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38, 1996. PubMed: 8707053 |
| Shipped： | freeze-dried |