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| 产品名称： | pMAM20 |
|---|---|
| 商品货号： | TS155989 |
| Designations： | pMAM20 |
| Species： | Strongylocentrotus purpuratus, purple sea urchin |
| Depositors： | B Polisky |
| Applications： | produces protein ectoderm protein (spec) |
| Vector： | Construct size (kb): 3.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3899999856948853 Tissue: embryo Gene product: ectoderm protein (spec) Target Gene: ectoderm protein (spec) |
| Insert Size (kb)： | 0.390 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | This clone encodes 5/6 of the spec1c gene. The depositor indicates its the specc6(1b) gene. The rop-spec fusion polypeptide is highly expressed at 42 C and can be used as a control for amplification of rop fusion polypeptides. |
| References： | Muesing M, et al. High-level expression in Escherichia coli of calcium-binding domains of an embryonic sea urchin protein. Gene 31: 155-164, 1984. PubMed: 6098520 |