| 产品名称： |
Chilodonella uncinata Ehrenberg |
| 商品货号： |
TS156013 |
| Deposited As： |
Chilodonella uncinata Ehrenberg |
| Strain Designations： |
ATCC:0189:1 |
| Biosafety Level： |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： |
Contaminant of Euplotes gracilis culture, ATCC 50191, 1988 |
| Product Format： |
frozen |
| Storage Conditions： |
Frozen: -70°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Protocols Section |
| Type Strain： |
no |
| Medium： |
ATCC® Medium 802: Sonneborns Paramecium medium
|
| Growth Conditions： |
Temperature: 25°C
Culture System:xa0Grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria |
| Cryopreservation： |
Reagents
Cryoprotective Solution
DMSO, 1.5 mL
Fresh growth medium w/o bacteria, 8.5 mL
Harvest and Preservation:
- Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.xa0 Allow to cool.
- Harvest cells from a culture in stationary phase (1-2 days after reaching peak density).
- Gently discard most of the supernatant and vigorously agitate the flasks to detach the cells.xa0
- Determine the cell concentration using a hemacytometer.xa0xa0 Adjust the concentration to 2 x 105/mL in fresh medium.xa0 If the concentration is too low, centrifuge at 200 x g for 5 minutes and resuspend the pellet with the supernatant to the desired volume.xa0
- Mix the cell preparation and the cryoprotective solution in equal portions.
- Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
- Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0
- Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.
- To establish a culture from the frozen place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.
- Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate the entire contents into a T-25 flask containing 10 mL of bacterized ATCC medium 802.
- Incubate at 25°C with the cap on loosely.
- Once the culture is established, follow the protocol for maintenance of culture.
|
| Name of Depositor： |
TA Nerad |
| Year of Origin： |
1988 |
| References： |
Grant JR, et al. Gene discovery from a pilot study of the transcriptomes from three diverse microbial eukaryotes: Corallomyxa tenera, Chilodonella uncinata, and Subulatomonas tetraspora. Protist Genomics 1: 3-18, 2012.
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