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微信小章| 产品名称： | p1-79 |
|---|---|
| 商品货号： | TS156032 |
| Designations： | p1-79 |
| Species： | Homo sapiens, human |
| Applications： | Studies of 3-generation Utah families suggest the numerous fragments homologous to this probe are inherited as haplotypes. Can use the entire plasmid as a probe. |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: DNA Segment, repetitive D1Z2 |
| Insert Size (kb)： | 0.900 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.1, 0.64; PstI--3.7; EcoRI--3.2, 0.64; HindIII--3.8; SmaI--3.8. The same locus was independently ascertained by Y. Nakamura. Studies of 3-generation Utah families suggest the numerous fragments homologous to this probe are inherited as haplotypes. This probe is a subclone of cosmid 2-30 from a human genomic library. Insert contains several copies of a 39 bp repeat. Variation in copy number may be basis for multiple alleles. TaqI reveals at least 50 fragments (from <1 to >50 kb), many of which are polymorphic. Each of 27 unrelated individuals tested showed distinct band pattern. Similar variation seen with HaeIII, MspI, PstI and PvuII. Can use the entire plasmid as a probe. Hybridizes with unusually high efficiency. |
| References： | Buroker N, et al. A hypervariable repeated sequence on human chromosome 1p36. Hum. Genet. 77: 175-181, 1987. PubMed: 2888721 Nakamura Y, et al. Characterization of a human midisatellite sequence. Nucleic Acids Res. 15: 2537-2547, 1987. PubMed: 3031603 Cogen PH, et al. Deletion mapping of the medulloblastoma locus on chromosome 17p. Genomics 8: 279-285, 1990. PubMed: 1979050 Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 Michael Litt, personal communication |