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| 产品名称： | pKSM710 |
|---|---|
| 商品货号： | TS156053 |
| Designations： | pKSM710 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0710000991821290 Vector: pKSM710 (phagemid) Promoters: Promoter for expression lambda PL Construction: pSM702, pMAMPF3 Marker(s):ampR,cmlR (am) Construct size (kb): 5.071000099182129 Features: insert detection: lacZalpha marker(s): ampR marker(s): cmlR (am) promoter for expression: lacUV5 promoter for expression: lambda PL promoter for in vitro transcription: T7lac replicon: fd replicon: pMB1 terminator: trpA transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | contains easily purifiable cassette(s) for construction expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--4.5, 0.5; PstI--5.0, DraIII--3.0, 2.1; DraIII/PvuI--2.0, 1.6, 0.9, 0.6. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--4.43; BglI--1.87; BglII--4.09; EcoRI--4.03, 4.48; NcoI--4.42; PstI--2.00; PvuI--2.13, 4.60; PvuII--3.40, 4.57. Expression vector containing a 2.57 kb BglII-BspHI cassette useful for constructing a variety of expression vectors. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |