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微信小章| 产品名称： | pbetaHalpha-5 pBHA-5 |
|---|---|
| 商品货号： | TS156055 |
| Designations： | pbetaHalpha-5 pBHA-5 |
| GenBank Number： | M16411 |
| Species： | Homo sapiens, human |
| Applications： | Five fragments have been used as probes: 1.4 kb KpnI, 0.31 kb HincII, 0.257 kb SacI, 0.569 kb EcoRI/SacI, and 0.388 kb PvuII/SacI. |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.944000005722046 Tissue: adult liver Gene product: hexosaminidase A (alpha polypeptide)( Hexose aminidase A (alpha polypeptide), beta-N-acetylhexosaminidase, N-acetyl-beta-glucosaminidase) HEXA Alleles: A2, A1, A1, A2 |
| Insert Size (kb)： | 1.944 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul, |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII--6.0; PstI--4.0, 2.0; AccI--4.8, 1.5; BamHI--6.0. Insert includes 168 bp 5 untranslated sequence, 1587 bp of open reading frame, and 186 bp 3 untranslated, encoding a protein of 529 amino acids, 60.7 kDa. There is a 5 deletion of 5-8 kb in a French-Canadian Tay-Sachs population. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Five fragments have been used as probes: 1.4 kb KpnI, 0.31 kb HincII, 0.257 kb SacI, 0.569 kb EcoRI/SacI, and 0.388 kb PvuII/SacI. |
| References： | Myerowitz R, Hogikyan ND. Different mutations in Ashkenazi Jewish and non-Jewish French Canadians with Tay-Sachs disease. Science 232: 1646-1648, 1986. PubMed: 3754980 Myerowitz R, et al. Human beta-hexosaminidase alpha chain: Coding sequence and homology with the beta chain. Proc. Natl. Acad. Sci. USA 82: 7830-7834, 1985. PubMed: 2933746 Nakai H, et al. Assignment of beta-hexosaminidase A alpha-subunit to human chromosomal region 15q24----q24. Cytogenet. Cell Genet. 56: 164, 1991. PubMed: 1829032 Myerowitz R. Splice junction mutation in some Ashkenazi Jews with Tay-Sachs disease: evidence against a single defect within this ethnic group. Proc. Natl. Acad. Sci. USA 85: 3955-3959, 1988. PubMed: 3375249 Barbara Weiffenbach, personal communication |