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| 产品名称： | pA11 A11 |
|---|---|
| 商品货号： | TS156159 |
| Designations： | pA11 A11 |
| Species： | Homo sapiens, human |
| Depositors： | GF Saunders |
| Vector： | Construct size (kb): 7.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 4.599999904632568 Tissue: DNA from human/hamster hybrid cell line (11p only) Gene product: DNA Segment, single copy D11S*1 |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 2.7; HindIII--7.3; BamHI--3.7 (doublet); PstI--3.7, 2.2, 1.1, 0.4; SalI--7.3. pA11 (TS156159/1) and pA33 (61582/3) detect adjacent NotI (500 and 375 kb, respectively) and SfiI (180 and 215 kb, respectively) fragments. This probe detects a 180 kb SfiI fragment and in lymphoblastoid cells, a 450 kb SalI fragment with no MluI sites, and a 275 SalI/NotI fragment. In fibroblastoid cells, it detects a 275 kb SalI fragment. The insert contains repetitive sequence. A PvuII fragment (about 0.45 kb) is "almost unique". |
| References： | Compton DA, et al. Definition of the limits of the Wilms tumor locus on human chromosome 11p13. Genomics 6: 309-315, 1990. PubMed: 2155176 Grady F Saunders, personal communication |