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| 产品名称： | lambdaNMT |
|---|---|
| 商品货号： | TS156193 |
| Designations： | lambdaNMT |
| Depositors： | H Okayama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 38.0000000000000000 Vector: lambdaNMT (phage, lambda - replacement) Construction: pSV2-neo, pcD, lambda762 Marker(s):G418R Construct size (kb): 38.0 Features: marker(s): G418R replicon: pMB1, SV40, lambda |
| Applications： | integrating vector vector for constructing cDNA libraries |
| Comments： | The suggested host for vector amplification is E. coli C600 (ATCC 23724) and for library amplification is E. coli JC8679 (ATCC 47001). Vector for efficient transduction of mammalian cells with a cDNA library constructed with the pcD (Okayama and Berg) expression vector. Cloning capacity: 12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector. The order of the major features in this bacteriophage is: lambda762/deltab - HindIII - 0.5 kb SV40 fragment, map units 0.86-0.95 - reddelta - SalI - SV40 ori - SV40 early promoter - SV40 late 19S and 16S splice junctions - neo (Tn5) - SV40 late poly(A) - pMB1 ori - ampR - lambda762/KH54/lambda ori/nin5/QSR. |
| References： | Okayama H, Berg P. Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells. Mol. Cell. Biol. 5: 1136-1142, 1985. PubMed: 3158804 |
| Shipped： | freeze-dried |