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| 产品名称： | pRY252 |
|---|---|
| 商品货号： | TS156194 |
| Designations： | pRY252 |
| Depositors： | RR Yocum |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli YMC9 |
| Vector Information： | Size (kb): 9.3000001907348630 Vector: pRY252 (plasmid) Promoters: Promoter CYC1 Construction: pBR322 Marker(s):ampR G418R URA3 Construct size (kb): 9.300000190734863 Features: marker(s): G418R marker(s): URA3 marker(s): ampR promoter: CYC1 replicon: 2 micron replicon: pMB1 |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 2.3; SalI--9.3; PvuII--uncut; XbaI--9.3; XhoI--8.9, 0.4. YE-type shuttle vector conferring G418 resistance under control of the CYC1 promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995 |
| Shipping Information： | Distributed: freeze-dried |