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| 产品名称： | pJQ210SK |
|---|---|
| 商品货号： | TS156210 |
| Designations： | pJQ210SK |
| Depositors： | MF Hynes |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1999998092651370 Vector: pJQ210SK (cosmid) Promoters: Promoter lac Construction: P15A ori, sacB, RP4, gtmR, cos, pBluescriptSK MCS Marker(s):sacB,gtmR Construct size (kb): 7.199999809265137 Features: insert detection: lac marker(s): gtmR, sacB promoter: lac replicon: P15A, oriT |
| Applications： | integrating vector vector for constructing genomic libraries vector permitting construction of fusion proteins vector permitting positive selection for integration vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): SstI--7.9; KpnI--6.0, 1.9; BamHI--7.9. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g. as a BamHI cassette). When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - cos - traJ - oriT - sacB. |
| Media： | ATCC® Medium 1885: LB medium (ATCC medium 1065) with 15 mcg/ml gentamycin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Quandt J, Hynes MF. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127: 15-21, 1993. PubMed: 8486283 |
| Shipped： | freeze-dried |