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| 产品名称： | pLACT7 |
|---|---|
| 商品货号： | TS156214 |
| Designations： | pLACT7 |
| Depositors： | GA Garcia |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.7789998054504400 Vector: pLACT7 (phagemid) Promoters: Promoter T7 (phi10) Construction: pTZ18U, pET21b Marker(s):ampR Construct size (kb): 4.77899980545044 Features: marker(s): ampR promoter: T7 (phi10) promoter: lac replicon: f1 replicon: pMB1 repressor gene: lacI MCS: NdeI...HindIII terminator: T7 (phi10) |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI-- 4.8; BglI--2.6, 1.3, 1.0; SacI--4.8. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.25; BglI--0.01, 2.47, 3.53; EcoRI--0.24, 0.30; EcoRV--1.86; HindIII--0.22; PvuI--0.05, 3.78; PvuII--0.08, 0.50, 2.01, 2.10; SacI--0.23; SalI--0.23; SphI--0.88. High copy number phagemid allowing controlled expression from either the T7 or the lac promoter. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Chong S, Garcia GA. A versatile and general prokaryotic expression vector, pLACT7. BioTechniques 17: 686-691, 1994. PubMed: 7833029 |
| Shipped： | freeze-dried |