| 产品名称: | pGEM4-Rb |
|---|---|
| 商品货号: | TS156229 |
| Designations: | pGEM4-Rb |
| GenBank Number: | U00113 |
| Species: | Gallus gallus, chicken |
| Depositors: | TD Gilmore |
| Vector: | Construct size (kb): 7.400000095367432 |
| Insert: | DNA: cDNA Insert lengths(kb): 4.5 Tissue: fibroblasts, embryo Gene product: retinoblastoma 1 RB1 |
| Insert Size (kb): | 4.5 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--2.9(doublet), 1.1, 0.62; EcoRI--3.0, 2.0, 1.6, 0.94; NotI--7.4. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.27; EcoRI--0.94, 2.54; HindIII--0.92, 1.62, 2.75, 3.16; KpnI--1.56; NcoI--1.59; NotI--0.27; PstI--1.85, 2.35, 2.86, 3.28; PvuII--3.24; SphI--1.09; XbaI-1.70. |
| References: | Feinstein R, et al. Characterization of a chicken cDNA encoding the retinoblastoma gene product. Biochim. Biophys. Acta 1218: 82-86, 1994. PubMed: 8193168 |