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| 产品名称： | pJAK15 JK389 |
|---|---|
| 商品货号： | TS156282 |
| Designations： | pJAK15 JK389 |
| Depositors： | JA Kornacki |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.0000000000000000 Vector: pJAK15 (plasmid) Promoters: Promoter tac Construction: pMMB67HE (ATCC 37623), Tn5 Marker(s):kanR (neoR) Construct size (kb): 11.0 Features: marker(s): kanR (neoR) promoter: tac replicon: RSF1010 replicon: oriT replicon: oriV repressor gene: lacIq MCS: HIndIII...EcoRI transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--11.0; PstI--8.2, 1.85, 1.0. A shuttle vector permitting autoregulated high-level expression in all gram-negative bacteria. Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Jon A Kornacki, personal communication |
| Related Products： | component of:ATCC 87055 |
| Shipped： | frozen |