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| 产品名称： | pLEX1B WM2184 |
|---|---|
| 商品货号： | TS156332 |
| Designations： | pLEX1B WM2184 |
| Depositors： | W Messer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.2909998893737790 Vector: pLEX1B (plasmid) Construction: pT7-7, pNM480 Marker(s):ampR Construct size (kb): 5.290999889373779 Features: marker(s): ampR replicon: pMB1 reporter group: lacZ MCS: XmnI...HindIII ribosome-binding site: Shine-Dalgarno |
| Applications： | expression vector vector permitting construction of fusion proteins beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; HindIII--5.3; PstI--5.3; XmnI--5.3. Expression vector with efficient prokaryotic expression signals upstream of the XmnI site. Permits generation of a translational fusion with lacZ. pLEX1B, pLEX2B, and pLEX3B (TS156332-87200) differ in the reading frame established at the XmnI site. Expression requires fusion of a 5TG of the insert with the A nucleotide of the XmnI site of the vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348 |
| Shipped： | freeze-dried |