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微信小章| 产品名称： | Canine parainfluenza virus |
|---|---|
| 商品货号： | TS156418 |
| Classification： | Paramyxoviridae, Paramyxovirinae, Unclassified |
| Agent： | Canine parainfluenza virus |
| Strain： | D008 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Antigenically very closely related to SV5.xa0 Hemagglutinates GP RBC. |
| Effect on Host： | CPE, cell rounding and degeneration |
| Recommended Host： |
D-17 cells (ATCC® CCL-183™)
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| Growth Conditions： | Temperature: 36°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3 to 6 days at 36°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | LN Binn |
| Source： | Throat swab from dog with respiratory disease |
| References： | Binn LN, et al. Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. Exp. Biol. Med. 126: 140-145, 1967. PubMed: 4294430 |