| 产品名称: | pUK |
|---|---|
| 商品货号: | TS156488 |
| Designations: | pUK |
| Depositors: | B Huang |
| Biosafety Level: | 1 |
| Vector Information: | Size (kb): 2.4300000667572020 Vector: pUK (plasmid) Promoters: Promoter tac Construction: pUC19, pKK223-3 Marker(s):ampR Construct size (kb): 2.430000066757202 Features: marker(s): ampR promoter: tac replicon: pMB1 MCS: HindIII-EcoRI ribosome-binding site: Shine-Dalgarno transcription terminator: rrnB |
| Applications: | expression vector |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; BamHI--2.4; HindIII--2.4. A small, high-copy-number vector suitable for both in vitro (using the coupled transcription/translation system of Zubay) and in vivo expression. |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions: | Temperature: 37.0°C |
| References: | Huang B, et al. A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151: 143-145, 1994. PubMed: 7828863 |
| Shipped: | freeze-dried |