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微信小章| 产品名称： | Rat Total RNA (NR8383) |
|---|---|
| 商品货号： | TS156490 |
| Product Format： | frozen |
| Storage Conditions： | -80°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Comments： | The cells exhibit characteristics of macrophage cells : phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst; IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors. NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta). Stimulation with bleomycin also increases TGF beta mRNA expression. These cells are sensitive to endotoxin. LPS levels of 1 to 10 ng/ml inhibit replication by 50%. LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/ml for extended periods. The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Quality Control Specifications： | The quality of the total RNA was determined using the Agilent 2100.xa0 RNA (0.1 –1 µL) was analyzed with a 6000 Pico LabChip Kit.xa0 The electropherogram shows high quality RNA with visible 18S and 28S rRNA peaks. |