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| 产品名称： | pHHH220 |
|---|---|
| 商品货号： | TS156516 |
| Designations： | pHHH220 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the TaqI RFLP was observed in 43 three-generation families. This probe has significant lane background, but RFLPs observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy D9S27 Alleles: A1, A2, A3 |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--7.6, 3.5, 0.8; BamHI--3.4, 2.4, 2.1, 1.7, 1.6; EcoRI/HindIII--2.5, 2.2, 1.8, 1.3 (doublet), 0.9, 0.8, 0.6, 0.4, 0.3; AccI--3.6, 3.5, 1.9 (doublet); EcoRI--4.7, 3.6, 1.8, 0.9, 0.6, 0.4; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pHHH220. The hybridization pattern of this probe with human DNA was not checked by the ATCC. Codominant segregation of the TaqI RFLP was observed in 43 three-generation families. This probe has significant lane background, but RFLPs observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI, PvuII and RsaI. |
| References： | Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence pHHH220 on chromosome 9p D9S27. Nucleic Acids Res. 15: 10606, 1987. PubMed: 2892176 |