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| 产品名称： | p134C |
|---|---|
| 商品货号： | TS156540 |
| Designations： | p134C |
| Species： | Homo sapiens, human |
| Depositors： | KJ Johnson |
| Applications： | The RFLPs are observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 3.089999914169312 |
| Insert： | DNA: genomic Insert lengths(kb): 0.3400000035762787 Tissue: 20XP3542-1-4 cell line Gene product: DNA Segment, single copy D19S51 Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 0.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.38; EcoRI--3.2; HindIII--3.2; AvaI--3.2; PstI--3.2. The BglI and PstI polymorphisms are in complete disequilibrium. The RFLPs are observed under normal hybridization and wash stringencies. p134C is linked to myotonic dystrophy (DM) with a LOD score in excess of 40 at theta = 0.006. It is distal to DM. The SmaI site was lost in ligation. Enzyme(s) not detecting polymorphism: TaqI, MspI, EcoRI, BamHI, HindIII, BglII, RsaI, PvuII, AluI, HinfI. |
| References： | Johnson K, et al. A new polymorphic probe which defines the region of chromosome 19 containing the myotonic dystrophy locus. Am. J. Hum. Genet. 46: 1073-1081, 1990. PubMed: 1971149 Tsilfidis C, et al. D19S51 is closely linked with and maps distal to the myotonic dystrophy locus on 19q. Am. J. Hum. Genet. 49: 961-965, 1991. PubMed: 1928101 |