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微信小章| 产品名称： | Human genomic DNA (HCC2218) |
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| 商品货号： | TS156551 |
| Product Format： | frozen Approx.10 µg |
| Storage Conditions： | -20°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Introduction： | Cell Line Description: The HCC2218 cell line was initiated on April 10, 1996, and took 6 months to establish. HCC2218 was derived from a female whose family members included several members with cancer, four lung cancer patients (two of them, non-smokers), two breast cancer patients (one of them also had lung cancer) and one colon cancer patient. HCC2218 is a poorly differentiated cell line derived from the primary tumor of an invasive ductal carcinoma with metastases in 42/43 lymph nodes. The cells are highly positive for expression of Her2/neu, and are also positive for expression of p53. HCC2218 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 EGP2 and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). DNA was isolated from an EBV-transformed lymphoblastoid cell line, HCC2218 BL, from the same patient is available as ATCC CRL-2363D. |
| Quality Control Specifications： | DNA Quality Control Information: ·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis ·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel ·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8 ·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion. |