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微信小章| 产品名称： | pHRed-102 |
|---|---|
| 商品货号： | TS156595 |
| Designations： | pHRed-102 |
| GenBank Number： | M11058 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.300000190734863 Tissue: fetal adrenal gland Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase HMGCR Alleles: A1, A2 |
| Insert Size (kb)： | 4.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert contains two internal BglII sites which will give a fragment (about 2.5 kb) from the insert. Restriction digests of the clone give the following sizes (kb): BglII--4.3, 2.8, 0.15; BamHI--4.2, 3.1, 0.1; SalI--7.3; PvuII--7.3; PstI--4.5, 1.75, 1.2. The insert includes the entire coding region and 50 bp 5 untranslated and 1.5 kb 3 untranslated sequences. Shows 87% homology with hamster cDNA in the coding region. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References： | Luskey KL, Stevens B. Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved domains responsible for catalytic activity and sterol-regulated degradation. J. Biol. Chem. 260: 10271-10277, 1985. PubMed: 2991281 Lindgren V, et al. Human genes involved in cholesterol metbolism: Chromosomal mapping of the loci for the low density lipoprotein receptor and 3-hydroxy-3-methylglutaryl-coenzyme A reductase with cDNA probes. Proc. Natl. Acad. Sci. USA 82: 8567-8571, 1985. PubMed: 3866240 Barbara Weiffenbach, personal communication |