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| 产品名称： | pBT7 |
|---|---|
| 商品货号： | TS156665 |
| Designations： | pBT7 |
| Depositors： | IN Maruyama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7539999485015870 Vector: pBT7 (plasmid) Promoters: Promoter lac Construction: pUC18, bacteriophage T7 Marker(s):ampR Construct size (kb): 2.753999948501587 Features: insert detection: lacZ marker(s): ampR promoter: lac, T7 gene 10 replicon: pMB1 |
| Applications： | expression vector vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): NdeI--2.75; HindIII--2.75; EcoRI--2.75. Expression vector based on a bacteriophage T7 RNA polymerase/promoter system and allowing visual detection of inserts using lacZalpha complementation. The T7 promoter sequence has a T->A mutation at the 5 end that does not appear to affect expression. The order and orientations of the major features of the plasmid are: - NdeI/MCS/EcoRI - |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maruyama IN, et al. A bacteriophage T7-based expression vector, pBT7, with color selection for the recombinant. Gene 131: 79-82, 1993. PubMed: 8370543 |
| Shipped： | freeze-dried |