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| 产品名称： | pBSL168 |
|---|---|
| 商品货号： | TS156702 |
| Designations： | pBSL168 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5 |
| Vector Information： | Size (kb): 3.3570001125335690 DESCRIPTION OF VECTOR COMPONENT: Name of vector: sBSL150 Intact vector size: 2.200 Type of vector: plasmid Vector end: mLuI Vector end: mLuI Cloning sites: Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI/EagI BstXI SacII SacI mLuI Construction: pSU18 Host range: Escherichia coli Features (with orientation and position when available): MCS: SacI...KpnI mLuI, -> MCS: mLuI KpnI...SacI, -> replicon: p15A marker(s): cmLR, Cross references: Vector: pBSL168 (plasmid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pSU18 Marker(s):ampR,cmlR Construct size (kb): 3.357000112533569 Features: marker(s): cmlR promoter for in vitro transcription: T7 (phi10) replicon: p15A MCS: KpnI...SacI MluI MCS: SacI...KpnI MluI coding sequence: bla (ampR) |
| Applications： | contains easily purifiable cassette(s) for construction wild type beta-lactamase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.0, 1.35; EcoRI--2.05, 1.25; MluI--2.1, 1.15; SstI--1.9, 1.4; NcoI/BamHI--1.4, 1.1, 0.9; NotI/ScaI--1.1, 0.9, 0.8, 0.7. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA in vitro. Contains a beta-lactamase (ampR) cassette flanked by multiple cloning sites duplicated in inverted orientation. The PstI and HincII sites in the bla gene have been removed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |