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| 产品名称： | 145-12 |
|---|---|
| 商品货号： | TS156773 |
| Designations： | 145-12 |
| Species： | Homo sapiens, human |
| Applications： | The RFLP-detecting clone is generated by double digestion of the insert with HindIII and EcoRI. The unique sequence fragments to be used for hybridization are the 500 bp doublet. |
| Vector： | Construct size (kb): 12.39999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 8.0 Gene product: DNA Segment, single copy DXS141 Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 8.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--12.4; PstI--6.2, 4.8, 1.4; BamHI--12.4; HindIII--8.0, 4.4; EcoRI--6.0, 3.9, 2.5. Part of a phage walk taken with the original pERT145 clone. This is one of the most proximal pERT clones absent from the BB deletion. The RFLP-detecting clone is generated by double digestion of the insert with HindIII and EcoRI. The unique sequence fragments to be used for hybridization are the 500 bp doublet. The remaining fragments are slightly repeated. |
| References： | Bertelson CJ, et al. Localisation of Xp21 meiotic exchange points in Duchenne muscular dystrophy families. J. Med. Genet. 23: 531-537, 1986. PubMed: 2879924 Kunkel LM, et al. Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc. Natl. Acad. Sci. USA 82: 4778-4782, 1985. PubMed: 2991893 Louis M Kunkel, personal communication |