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| 产品名称： | III |
|---|---|
| 商品货号： | TS156806 |
| Designations： | III |
| Species： | Homo sapiens, human |
| Depositors： | JE Sadler |
| Vector： | Construct size (kb): 6.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.0 Tissue: umbilical vein endothelium cell line Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) VWF Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--4.0, 2.7; EcoRI--6.7; BamHI--6.7; PstI--3.6, 1.9, 0.8, 0.3; HindIII--6.7. Probes I (ATCC 59782/59783), II (ATCC 59784/59785), III (TS156806/59787) and IV (ATCC 59788/59789) constitute non-overlapping fragments of the human von Willebrand factor cDNA sequence that together span essentially the entire 9 kb cDNA. The 2.75 kb FspI/SacI portion of the insert constitutes probe III and corresponds to amino acids 544-1461 of von Willebrand factor. Detects the gene on chromosome 12 and a pseudogene on chromosome 22. Insert is the 5 4 kb SacI(vector)/SacI fragment of lambda HvWF3. |
| References： | Shelton-Inloes BB, et al. Evolution of human von Willebrand Factor: cDNA sequence polymorphisms, repeated domains, and relationship to von Willebrand Antigen II. Biochem. Biophys. Res. Commun. 144: 657-665, 1987. PubMed: 3495266 Shelton-Inloes BB, et al. Gene deletions correlate with the development of alloantibodies in von Willebrand disease. J. Clin. Invest. 79: 1459-1465, 1987. PubMed: 3033024 J E Sadler, personal communication |