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微信小章| 产品名称： | MIA PaCa-2 |
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| 商品货号： | TS156821 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Cell Type： | epithelial cell (KRAS CRM) |
| Product Format： | frozen |
| Morphology： | attached epithelial with floating rounded cells |
| Culture Properties： | adherent, single cells and loosely attached clusters |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 65 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Applications： | For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.xa0 |
| Intended Use： | Certified Reference Material produced under an ISO 17034 accredited process.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent. |
| Derivation： | The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1). |
| Clinical Data： | 65 years Caucasian male |
| Genes Expressed： | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
| Cellular Products： | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
| Comments： | Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process. Item has been tested for KRAS mutation (p.G12C c.34G>T) The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
The line is sensitive to asparaginase. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
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| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium 95%; DMSO, 5% Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 12,13 D16S539: 10,13 D5S818: 12,13 D7S820: 12,13 THO1: 9,10 TPOX: 9 vWA: 15 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | A Yunis |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590 Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740 Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455 Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918 |
