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| 产品名称： | pRS421 |
|---|---|
| 商品货号： | TS156825 |
| Designations： | pRS421 |
| Depositors： | JD Boeke, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size of construct (kb): 6.233 Markers: ampR, MET15 DNA Seq. Accession number: U93716 Type of vector: phagemid Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalI XhoI ApaI KpnI Construction: pRS401, YEp24 Host range: S. cerevisiae; E. coli Features (with position when available): marker(s): MET15, 421-1270 replicon: f1, 1860-2316 promoter for in vitro transcription: T7, 2486-2505 MCS: KpnI...SacI, 2513-2615 promoter for in vitro transcription: T3, 2632-2651 insert detection: lacZ, 2317-2676 promoter: lac, 2721-2749 replicon: pMB1, 3075-3075 marker(s): ampR, 3833-4693 replicon: 2 micron, 4825-6166 |
| Applications： | YE-type (episomal) shuttle vector
Vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | Component of: ATCC 87476 Component of: ATCC 87529 Component of: ATCC 87535 |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of Escherichia coli containing the plasmid |